Assemblytics

Upload a delta file to analyze alignments of an assembly to another assembly or a reference genome

1. Download and install MUMmer
2. Align your assembly to a reference genome using nucmer (from MUMmer package)

   $ nucmer -maxmatch -l 100 -c 500 REFERENCE.fa ASSEMBLY.fa -prefix OUT

   Consult the MUMmer manual if you encounter problems

3. Optional: Gzip the delta file to speed up upload (usually 2-4X faster)

   $ gzip OUT.delta

   Then use the OUT.delta.gz file for upload.

4. Upload the .delta or delta.gz file (view example) to Assemblytics

Important: Use only contigs rather than scaffolds from the assembly. This will prevent false positives when the number of Ns in the scaffolded sequence does not match perfectly to the distance in the reference.

The unique sequence length required represents an anchor for determining if a sequence is unique enough to safely call variants from, which is an alternative to the mapping quality filter for read alignment.